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. 2019 May 10;8(5):31. doi: 10.1038/s41389-019-0143-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Subcellular fractionation analyses examining abundance of nuclear and cytoplasmic YAP protein expression in SW-1990 PDAC cells stimulated with hypoxia in the indicated times. GAPDH and Lamin B were used as internal control of cytoplasmic and nuclear extractions, respectively. SF serum-free, N normoxia, H hypoxia, WB western-blotting, NE nuclear extraction, CE cytoplasmic extraction, WCE whole cell extraction. Data are expressed as mean ± s.d. of three independent experiments. *P < 0.05. Two-sided Student’s t test was used to calculate the P value. b Representative immunfluorescence images of nuclear YAP localization in SW-1990 PDAC cells stimulated with hypoxia in the presence or absence of Flag-tagged wild-type 14-3-3ζ transfection for 6 h. Scale bar = 25 μm. c Subcellular fractionation analyses detecting abundance of nuclear and cytoplasmic YAP protein expression in hypoxia-stimulated SW-1990 PDAC cells with or without Flag-tagged wild-type 14-3-3ζ transfection. d Coimmunoprecipitation assay evaluating the interaction between 14-3-3ζ and YAP in SW-1990 PDAC cells stimulated with hypoxia for 6 h. Data are expressed as mean ± s.d. of three independent experiments. *P < 0.05. Two-sided ANOVA with Bonferroni post hoc t test correction was used to calculate the P value