Skip to main content
. 2019 Jul;59:34–40. doi: 10.1016/j.cellsig.2019.03.015

Fig. 2.

Fig. 2

Concentration-dependent inhibition of platelet function by RBC8 in murine RalAB double knockout (DKO) platelets. (A and B) Washed platelets (2 × 108/mL) from WT and DKO mice were pretreated with indicated concentrations of RBC8 or vehicle (0.2% DMSO) and assessed for platelet aggregation (A) and dense granule secretion (B) by lumi-aggregometry using a threshold concentration of CRP (0.6 μg/mL). (C and D) Following pretreatment with vehicle or RBC8, washed platelets (2 × 107/mL) were stimulated with indicated concentrations of CRP or PAR-4 for 10 min and monitored for integrin αIIbβ3 activation (C) and α-granule secretion of P-selectin (D) by flow cytometry using a JON/A and P-selectin antibody, respectively. Data are mean ± s.d., n = 5, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. indicated sample.