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. 2019 Mar 5;8(1):1–20. doi: 10.1016/j.jcmgh.2019.02.007

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

IEC (IPEC-J2) monolayers express IFN-β but not IFN-α mRNA in response to C parvum infection. qRT-PCR analysis of (A) IFN-α (all) isotypes, (B) IFN-α7/11 isotypes, (C) IFN-α9 isotype, and (D) IFN-β during a 48-hour time course of C parvum infection in IPEC-J2 cells. N = 4 monolayers per treatment group and time point. For each monolayer, the Ct for target gene expression was normalized to expression of the housekeeping gene cyclophilin (ΔCt). Fold-change differences between uninfected and infected monolayers are expressed as compared with a representative uninfected monolayer at the same time point using the 2^-ΔΔCt method. Bars represent means ± SD. *P < .05, **P < .01, Student t test comparison between uninfected and infected monolayers at same time point. Results were obtained from 1 independent experiment.