Table 1.
Specific Primer Sequences and Sources Used in Real-Time RT-PCR and Their Resulting Amplification Product Sizes and Melting Temperatures
| mRNA | Forward, 5’ to 3’ | Reverse, 5’ to 3’ | Product size | Melting temperature, C° | Reference |
|---|---|---|---|---|---|
| Cp gp40/15 | TCATTTGTAATGTGGTTCGGAGAA | AGGGTAAAGGCAAACAAATCG | 326 | 82.5–83.0 | 48 |
| Porcine-IFNα (all) | GGCTCTGGTGCATGAGATGC | CAGCCAGGATGGAGTCCTCC | 197 | 88.45–88.80 | 15 |
| Porcine-IFNα 7/11 | GGGACTTTGGATCCCCTCAT | GTGGAGGAAGAGAAGGATG | 369 | 89.0–89.5 | 49 |
| Porcine-IFNα9 | GTGCTGCTCAGCTGCAAG | AGTCCTCCTCCAGCAGGGGC | 384 | 87.5–88.5 | 49 |
| Porcine-IFNβ | ATGTCAGAAGCTCCTGGGACAGTT | AGGTCATCCATCTGCCCATCAAGT | 246 | 82.20–82.70 | 15 |
| Porcine-IFNγ | GTTTTTCTGGCTCTTACTGC | CTTCCGCTTTCTTAGGTTAG | 410 | 86.3–87.0 | 50 |
| Porcine-IFNλ1 | ACATCCACGTCGAACTTCAGGCTT | TCAGATGTGCAAGTCTCCACTGGT | 209 | 87.5–88.0 | 49 |
| Porcine-IFNλ3 | AAGAGGGCCAAGGATGCCTTTGAA | AGGCGGAAGAGGTTGAACATGACA | 374 | 92.9–93.3 | 49 |
| Porcine-IFNλ4 | GTCACAGAGCTGACTCGCCT | TCACAGACAAGGCCCCGAAT | 110 | 89.2–89.5 | Authors |
| Porcine-ISG15 | GATGCTGGGAGGCAAGGA | CAGGATGCTCAGTGGGTCTCT | 229 | 90.0–90.3 | Authors |
| Porcine-IFNλR1 | CGGTGGCAAAGAGTGAAAAT | GATCTCCTCTGTCCGGGTGA | 249 | 86.7–86.8 | Authors |
| Porcine-Cyclophilin | CCGTCGATGGCGAGCCC | CCCGTATGCTTCAGGATAAAA | 250 | 82.5–84.5 | 51 |
| Murine-IFNL2/3 | AGCTGCAGGCCTTCAAAAAG | TGGGAGTGAATGTGGCTCAG | 244 | 88.5–89.1 | 7 |
| Murine-ISG15 | GAGCTAGAGCCTGCAGCAAT | TTCTGGGCAATCTGCTTCTT | 122 | 84.3–84.5 | 7 |
| Murine-IFNβ | GGAGATGACGGAGAAGATGC | CCCAGTGCTGGAGAAATTGT | 103 | 79.9–80.6 | 52 |
| Murine-GAPDH | TGCACCACCAACTGCTTAGC | GGCATGGACTGTGGTCATGAG | 87 | 84.2–84.4 | 7 |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase.