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. 2019 Mar 1;8(1):21–36. doi: 10.1016/j.jcmgh.2019.02.006

Figure 1.

Figure 1

REG3γ expression in IEC was impaired in IL33-/-mice. (A–C) IECs were isolated from small intestines of WT and IL33-/- mice (n = 4/group). The expression of REG3γ was determined by qRT-PCR and normalized against glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (GAPDH) (A), and by Western blot, with β-actin as the loading control (B). The expression of β-defensin-1, -2, -3, and -4 was determined by qRT-PCR and normalized against GAPDH (C). (D–F) WT mice (n = 3/group) were fed with 1.65% (wt/vol) DSS in drinking water for 7 days and water alone for additional 3 days to induce colitis. IL33 expression in IEC of IL33-/- mice, WT mice, and colitic WT mice with Dextran sulfate sodium (DSS) was measured by qRT-PCR and normalized against GAPDH (D), enzyme-linked immunosorbent assay (E), and Western blot (F). Significance was calculated using an unpaired Student t test. *P < .05, **P < .01. Data are represented as means ± standard deviation. One representative of 3 experiments with similar results.