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. 2019 Mar 1;8(1):21–36. doi: 10.1016/j.jcmgh.2019.02.006

Figure 5.

Figure 5

IL33 induction of REG3γ is not dependent on NF-kB activation, and IL33 has no effect on SOCS3, HIF1α, and ST2 expression in IEC. (A) MSIE cells (n = 3 wells/group) were treated with 10 ng/mL of IL33 in the presence or absence of NF-κB inhibitor, Bay 11-7082. The expression of REG3γ was determined by qRT-PCR and normalized against glyceraldehyde-3-phosphate dehydrogenase (phosphorylating). (B–D) MSIE cells (n = 3 wells/group) were treated with IL33 (10 ng/mL) at different time points. The expression of SOCS3 (B), HIF1α (C), and ST2 (D) were determined by qRT-PCR and normalized against glyceraldehyde-3-phosphate dehydrogenase (phosphorylating). Significance was calculated by 1-way analysis of variance test. *P < .05. Data are represented as means ± standard deviation. One representative of 3–4 experiments with similar results.