Skip to main content
. 2019 May 7;12(7):871–878. doi: 10.1016/j.tranon.2019.04.007

Figure 2.

Figure 2

Assessment of olaparib mechanistic function in TaxR vs. C4-2B cells. (A) C4-2B and TaxR cells were treated with increasing doses of olaparib for 30 minutes. Cells were then harvested and whole cell lysates were subjected to Western blot for PAR (upper panel). GAPDH served as a loading control. Densitometric quantification of the blot is shown (lower panel). PAR levels are normalized to GAPDH expression. (B) C4-2B and TaxR cells were treated with 0.01% MMS and indicated doses of olaparib for 4 hours and then harvested and fractionated to isolate the chromatin-bound fraction. Chromatin-bound fraction was subjected to Western blot for PARP. Histone-H3 served as a loading control. Ola = olaparib, Ab = antibody used.