Fig. 6.

Spatial proximity and interchromosomal trans SSA repair frequency do not correlate. (A) Schematic of single-strand annealing (SSA) assay showing ADE2 suicide deletion cassette and LEU2 cassettes with I-SceI cut sites flanked by 28-bp direct repeats. (B) Diagram showing the fixed position of the ADE2 cassette on chrXV and variable positions of the LEU2 cassette. (C) Absolute survival (galactose plates). Non-SSA outcomes (e.g., precise NHEJ) were scored as colonies that had successfully deleted the URA-marked suicide deletion cassette to become Ura− but that had not converted to either the Ade+ (cis SSA) or Leu+ (trans SSA) phenotypes. (D) Lack of correlation between trans repair and contact frequencies (galactose plate method). (E) Lack of correlation between trans repair and contact frequencies (liquid galactose method). Data represent the mean ± SD from four independent experiments. ns, not significant.