Fig. 5.

Investigation of the role of ZIKV host factors in human astrocytes. (A and B) Human astrocytes derived from iPS-wt5 were susceptible to ZIKV^U (A) and ZIKV^PR (B) infection. Astrocytes were infected with multiplicity of infection (MOI) 0.1 ZIKV^U or MOI 0.6 ZIKV^PR, and collected at 48 h. (Scale bars: 1,000 μm.) (C and D) Astrocytes targeted with individual sgRNAs show varying degrees of infection by ZIKV^U (C) and ZIKV^PR (D), as measured by viral RNA release into the medium. Astrocytes were infected with MOI 1 ZIKV^U or ZIKV^PR and collected at 72 h. Experimental sgRNA results that showed significant reduction compared with control sgRNA were noted. Two technical replicates. Results are mean ± SEM. *P < 0.05, ***P < 0.001, **P < 0.01, ****P < 0.0001.