Fig. 4.
Pnpla3 knockdown ameliorates fatty liver in the Pnpla3148M/M mice. Male Pnpla3+/+ and Pnpla3148M/M mice (n = 7 per group, aged 7 to 9 wk) were injected with AAV-expressing anti-Pnpla3 shRNA (2.25 × 1011 GCs). The mice were fed a high-fructose diet for 2 wk before dietary synchronization. Livers were harvested and LDs were prepared as described in SI Appendix, Methods. LD proteins (4 μg) were analyzed by immunoblotting using an anti-rabbit mAb (19A6) and guinea pig anti-mouse PLIN2 polyclonal Ab (Methods). Each bar represents mean ± SEM values. *P < 0.05, ***P < 0.001. The experiment was repeated and the results were similar.