Fig. 7.

Speculation on the mechanisms by which binding multiple PIP₃ molecules stabilizes the Saraste dimer on the membrane. (A) Fluctuations at the Saraste dimer interface region in an individual PH–TH module in conventional MD simulations in solution and on the membrane. (B) An instantaneous structure from a tempered binding simulation showing that a PIP₃ lipid can simultaneously interact with both modules in the Saraste dimer at the bridging site, which formed near the dimer interface.