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. Author manuscript; available in PMC: 2019 May 11.
Published in final edited form as: ACS Nano. 2018 Dec 14;13(1):203–214. doi: 10.1021/acsnano.8b05286

Figure 6: Cell traction force and adhesion.

Figure 6:

Cell substrate interaction is determined by traction force measurement and focal adhesion. Huh7 cells are grown on HA and 30 kPa substrates for 24 hrs. (a) (left) Bright field image of the cell; (middle) marge image of cell (blue), stressed beads(red) and relaxed beads(green); (right) bead displacement towards the center of the cell. (Scale bar, 10 μm). (b) Bead displacement fields are shown for the cells on HA and 30 kPa. (c) Average net contractile moment and strain energy are shown. (N= 7 and 11 cells respectively for HA and 30 kPa substrates). (d) Immunostaining images of Paxillin (green) are shown. Intensity thresholded image and Focal adhesion boundaries are shown (Scale bar, 10 μm). (e) Average Focal adhesion area, average number of FAs (N= 15 and 16 cells on HA and 30 kPa respectively) and FA length are plotted on HA and 30kPa substrate. All the FA areas and lengths are normalized by the individual cell area. Error bars are standard error mean. p-values are determined from Student’s t-test for unpaired samples f (** p<0.005; * p<0.05).