Fig. 3.

Downregulation of UBN2 represses human CRC cells proliferation and tumorigenesis. a The protein expression of UBN2 in CRC cell lines (α-tubulin as an internal reference). b Western blot was performed in SW837 and LoVo cells. SW837 and LoVo cells were transfected with vector (NC) or inhibitors (UBN2-S1, UBN2-S2) of UBN2. c–e Cell proliferation was determined by using MTT (left, factorial analysis, p < 0.01) (c), colony formation assays (p < 0.01) (d), and soft agar assay (p < 0.01) (e). Scale bars: 20 µm. f, g. SW837/vector and SW837/shUBN2 cells (2 × 10⁶) were injected subcutaneously into nude mice (n = 7). Tumors were collected and measured after the mice were sacrificed (f). Lines within boxes and whiskers represent medians and extreme, respectively. All tissues taken from nude mice were subjected to H&E or immunohistochemical staining (Ki-67). Histogram analysis of the percentage of Ki-67-positive cells (g). Scale bars: 50 µm. Error bars represent mean ± SD from three independent experiments. **p < 0.01