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. 2019 May 10;20:236. doi: 10.1186/s12859-019-2854-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Time consumption of Alientrimmer, cutPrimers pTrimmer and BAMClipper with the increasing depth from 1000× to 10,000×. The results of Cutadapt were excluded due to its huge time consumption. And the results of BAMClipper is the running time of processing the bam files. For better displaying, we run Alientrimmer, BAMClipper and pTrimmer with one thread and run cutPrimers with 8 threads