Fig. 7.

Effects of melatonin on the expression of the ER stress marker genes GRP78 and CHOP during PA-induced mouse granulosa cell apoptosis after treatment for 24 h. a Western blot assays of GRP78 and CHOP expression after treatment with different concentrations of melatonin (0.1-100 μM) and 400 μM PA for 24 h. b Relative GRP78 expression in mouse granulosa cells after treatment with different concentrations of melatonin (0.1-100 μM) and 400 μM PA for 24 h. c Relative CHOP expression in H9C2 cells after treatment with different concentrations of melatonin (0.1-100 μM) and 400 μM PA for 24 h. GRP78 and CHOP expression were normalized to the level of β-actin expression. The Statistical analysis results are shown in the bar graphs. Data are presented as the mean ± SEM of three independent experiments. Bars with different letters are significantly different (p < 0.05)