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. Author manuscript; available in PMC: 2019 Jun 5.
Published in final edited form as: Cell Rep. 2018 Jun 5;23(10):3091–3101. doi: 10.1016/j.celrep.2018.04.116

Figure 5. LI71 Inhibits LIN28 by Competing for the RNA Binding Site in the CSD.

Figure 5.

(A) LI71 inhibits the interactions between LIN28 and preE-let-7a, preE-let-7d, preE-let-7f, and preE-let-7g. The IC50 of LI71 in our primary fluorescence polarization assay is ~7 μM. The IC50 of LI71 in oligouridylation assay is ~27 μM.

(B) Surface presentation of CSD and saturation transfer difference (STD) spectrum of LI71 in the presence of LIN28, preE-let-7, and LIN28 variants. When LI71 is in close contact with an irradiated bio-molecule, saturation transfer difference peaks can be observed.

(C) Dose-response oligouridylation assay for LI71. *pre-let-7g; **oligouridylated pre-let-7g. The bottom band intensity is measured and plotted. LI71 shows an IC50 of 27 μM.

(D) LI71 variants and their IC50s determined from dose-response fluorescence polarization assay.