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. Author manuscript; available in PMC: 2019 Jun 5.
Published in final edited form as: Cell Rep. 2018 Jun 5;23(10):3091–3101. doi: 10.1016/j.celrep.2018.04.116

Figure 7. 5-(Methylamino)nicotinic Acid Presents a Minimal Scaffold to Inhibit LIN28.

Figure 7.

(A) Dose-response fluorescence polarization assay of 5-(methylamino)nicotinic acid (MNA) (LI101) shows an IC50 of 76 μM (n = 3 replicates). Error bars denote mean ± SEM.

(B) Oligouridylation assay with positive control (RNA), negative control (DMSO), and 5-(methyl-amino)nicotinic acid. *Oliouridylated pre-let-7g.

(C) HeLa clones expressing the dual luciferase let-7 sensor were incubated with either vehicle (DMSO) or 5-(methylamino)nicotinic acid at various concentrations (50 μM, 100 μM, 150 μM, 250 μM, and 500 μM) for 48 hr, at which time luciferase activity was measured (n = 5 replicates; *p < 0.0001). Error bars denote mean ± SEM.