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Real-time PCR primers used for mRNA expression analysis
| Target gene | Prime(5ʹ-3ʹ) |
|---|---|
| U6 | F- TCCGATCGTGAAGCGTTC R- GTGCAGGGTCCGAGGT |
| miR-506-3p | F- GCCACCACCATCAGCCATAC R- GCACATTACTCTACTCAGAAGGG |
| HOXA11-AS | F- AGAAATCTGGACCCGAGACG R- CGTCAGCTTACGTCTCCAAA |
| NEK6 | F- TTCCAACAACCTCTGCCACACC R- CACAGTCCTGCCTCGCCTTG |
| GAPDH | F- AAGGTGAAGGTCGGAGTCAA R- -AATGAAGGGGTCATTGATGG |
Abbreviations: F, forward; mRNA, messenger RNA; PCR, polymerase chain reaction; R, reverse.
