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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Apr 4;190:183–192. doi: 10.1016/j.jsbmb.2019.04.003

Figure 4: Mutation of SP1 sites blunts the activation of the LHCGR promoter.

Figure 4:

hLGCs were plated for 24 h in serum-free media followed by a 48-hour incubation with a lentiviral vector carrying a control reporter plasmid, a plasmid containing an intact proximal human LHCGR promoter, or a plasmid containing mutations in the SP1 sites of the LHCGR promoter. Cells were harvested after no treatment (C) or treatment with hCG (50 ng/ml), forskolin (5 μM), or dbcAMP (1 mM) for 48 h. Quantitation of promoter activity was performed using a luciferase reporter assay. *P < 0.05 t-test, n = 6.