miR-200c-3p expression is frequently decreased in PCa. (A) Expression of miR-200c-3p by qRT-PCR in 22 pairs of PCa tissues and normal tissues; (B) expression of miR-200c-3p in PCa cell lines (PC3 and DU145), compared with RWPE-1 cell (human prostatic epithelial cell line); (C) miR-200c-3p was positively correlated with PCa pathologic T stage (the total number of enrolled cases from TCGA database is 487, with a P value of 1.667e-01 by the Kruskal-Wallis test); (D) miR-200c-3p was negatively related to PCa pathologic N stage (the total number of enrolled cases from TCGA database was 422, with a P value of 2.618e-01 by the Wilcox test); (E) miR-200c-3p was not significantly associated with overall survival in PCa (0, low miR-200c-3p group with the sample number of 246; 1, high miR-200c-3p group with the sample number of 245, HR for miR-200c-3p =0.68, with a P value of 6.399e-01 by the Cox regression test). ***, P<0.001. PCa, prostate cancer.