Fig. 6.

a: Amplification of 18srRNA gene of Plasmodium using the freeze-thaw samples. M-100 bp marker. 1-30 are samples of freeze-thaw in sterile distilled water and phosphate buffer saline at different freezing temperature (−80°C, −20°C and 0°C) respectively, 31-positive control and 32- negative control

b: Amplification of mitochondrial gene of Plasmodium using the freeze-thaw samples M-100 bp marker. 1–30 are samples of freeze-thaw in sterile distilled water and phosphate buffer saline at different freezing temperature (−80°C, −20°C and 0°C ) respectively, 31-positive control and 32- negative control

c: Showing the amplification of single copy gene msp1 and msp2 and its alleles using freeze-thaw samples thaw at −80°C. Lane 1 for D allele=D1, Lane 2=D2, Lane 3=D3, Lane 4=D4, Lane 5=SDW, Lane 6=D6, Lane 7 F allele= F1, Lane 8=F2, Lane 9=F3, Lane 10= F4, Lane 11=SDW, Lane 12=F5, Lane 13 for K allele=K1, Lane 14=K2, Lane 15=K3, Lane 16=K4, Lane 17=SDW, Lane 18=K5, Lane 19 for R allele=R1, Lane 20=R2, Lane 21=R3, Lane 22=R4, Lane 23=SDW, Lane 24=R5, Lane 25 for M allele=M1, Lane 26=M2, Lane 27=M3, Lane 28=M4, Lane 29=SDW, Lane 30=M5. D=IC3D7 allele, F=FC27 allele, K=K1 allele, R=R033 allele and M=MAD20 allele, M-100 bp marker