Figure 3.

Effect of 24, 48 and 96 h incubation with serum-supplemented media (SSM; A) and prostate-conditioned media (PCM; B) from sedentary (SED; n = 6-7) and exercise-trained (ET; n = 8-9) Copenhagen rats on migration of AT-1 cells. A representative image of cell migration utilizing a cell exclusion zone technique in a 96-well tissue culture plate is illustrated to the right of each bar graph. No significant difference in cell migration between SED and ET groups was observed in SSM or PCM from 24-96 h (P > 0.05). Bars with different letters are statistically different from one another within SED group over time. Bars with different numbers are statistically different from one another within ET group over time. Values are expressed as mean cell number ± SEM. P ≤ 0.05 for 24 vs. 48, 24 vs. 96, and 48 vs. 96 h within SED or ET group.