Figure 10.
Effect of 24 h incubation with serum-supplemented media from tumor-bearing rats (SSM-TB; A) and prostate-conditioned media from tumor-bearing rats (PCM-TB; B) from sedentary tumor-bearing (SED-TB; n = 3-4) and exercise-trained tumor-bearing (ET-TB; n = 4) Nude rats on migration (via Transwell migration assay) of PC-3 cells in normoxia. No significant difference in cell migration between groups was observed (P > 0.05). Values are expressed as mean cell count/total area (pixels2) ± SEM. Bar graph representing the percentage of PC-3 cells in G0/G1, S, and G2/M cell cycle phase after treatment of PC-3 cells with serum-supplemented media from tumor-bearing rats (SSM-TB; C) and prostate-conditioned media from tumor-bearing rats (PCM-TB; D) from sedentary tumor-bearing (SED-TB; n = 4) and exercise-trained tumor-bearing (ET-TB; n = 4) Nude rats in normoxia. There was no significant effect of SSM-TB on cell population in different cell cycle phase in SED-TB vs. ET-TB rats (P > 0.05). PCM-TB however caused a significant decrease of cells in S phase in ET-TB vs. SED-TB (P ≤ 0.05). Values are expressed as mean proportion of cells (%) ± SEM. *P ≤ 0.05 vs. SED-TB, within PCM-TB.