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. 2019 May 6;11:4023–4040. doi: 10.2147/CMAR.S198886

Figure 2.

Figure 2

miR-107 delivery into HLA-DRCD33+MDSCs via TDEs secreted by gastric cancer cells.

Notes: HLA-DRCD33+MDSCs were incubated with TDEs containing Cy3-labeled miR-107 for 6 hrs prior to examination for miR-107 internalization. Shown here are Wright stained image, magnification × 400, (A), CFSE proliferation dye, magnification × 100 (B, Cy3-miR-107 , magnification × 400 (C), and a merged image of CFSE and Cy3, magnification × 100 (D) showing internalization of miR-107 into proliferating HLA-DRCD33+MDSCs. (E) HLA-DRCD33+MDSCs were incubated with TDEs containing miR-107 for 24 hrs prior to collection for real-time quantitative PCR analysis. Shown here is relative expression of miR-107 and pre-miR-107 in the MDSCs. The data are presented as mean ± SD (n=6). **P<0.01.