Figure 2.

GdnCl denaturation of CcdB under two different buffer conditions. Equilibrium denaturation profiles of CcdB in 10 mM HEPES, (•) pH 8.4 and 200 mM HEPES (▾), pH 8.4 at 25 °C using both fluorimeter and nanoDSF. Isothermal melts were carried out using 47 μg/mL (2 μM) protein in both cases. The experimental data are shown in dots or inverted triangles, while the fits are shown as smooth lines. The theoretical curves were obtained by fitting all the melts with two‐state unfolding models (N₂↔2D) as described previously.7