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. 2019 Apr 29;28(6):1127–1134. doi: 10.1002/pro.3622

Figure 5.

Figure 5

Measurement reproducibility and determination of sample sensitivity of nanoDSF using two different sets of capillaries for 48 different denaturant concentrations. Equilibrium GdnCl denaturation profiles of two different concentration of HEWL (a) 15 μg/mL and (b) 7.5 μg/mL in PBS, pH 7.5 at 25 °C using nanoDSF. Two set of capillaries [capillary 1 (Inline graphic) and capillary 2 (Inline graphic)] were used to examine the reproducibility of the data. The data points in denaturation profiles using 7.5 μg/mL of HEWL show increased scatter as compared to data for 15 μg/mL.