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Absence of photobleaching of samples during measurement time scale. The intensity of 50 μg/mL of CcdB using two different capillaries was monitored for a period of 700 s at 100% excitation power at 20 °C (c) to detect any visible photobleaching of intrinsic fluorophores present in the protein samples, during the time course of measurement. The unfolding (a) and refolding (b) of the same sample is also plotted in terms of ratio of fluorescence at 350/330 nm in the same time window.
