Table 1.
Thermodynamic Parameters of CcdB and Lysozyme Measured under Different Buffer Conditionsa
| Proteins | Buffer | Fluorimeter | nanoDSF | ||||
|---|---|---|---|---|---|---|---|
| ΔG0 (kcal.mol−1) | m (kcal.mol−1 M−1) | Cm (M) | ΔG0 (kcal.mol−1) | m (kcal.mol−1 M−1) | Cm (M) | ||
| CcdB | 10 mM HEPES, pH 8.4 | 22.2 ± 0.08 | 4.89 ± 0.04 | 2.49 ± 0.05 | 22.4 ± 0.09 | 4.94 ± 0.06 | 2.51 ± 0.02 |
| CcdB | 200 mM HEPES, pH 8.4 | 23.7 ± 0.07 | 4.60 ± 0.05 | 3.03 ± 0.05 | 23.9 ± 0.05 | 4.48 ± 0.06 | 3.2 ± 0.1 |
| Lysozyme | 1 M Urea, PBS, pH 7.5 | 7.7 ± 0.07 | 2.80 ± 0.05 | 3.33 ± 0.05 | 7.5 ± 0.05 | 2.67 ± 0.06 | 3.35 ± 0.1 |
| Lysozyme | 2 M Urea, PBS, pH 7.5 | 6.9 ± 0.05 | 2.10 ± 0.05 | 2.94 ± 0.05 | 7.1 ± 0.08 | 2.3 ± 0.06 | 2.93 ± 0.02 |
a
Reported standard errors are derived from two independent experiments.