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Sodium dodecly sulfate ‐ polyacrylamide gel electrophoresis (SDS‐PAGE) analysis of steps in the production and purification of RALF8. “TOTAL” is from cell lysate. “LOAD” represents the material loaded onto the IMAC column. “WASH 1” and “WASH 2” are from successive column washes. “ELUTE” is from the eluate. “SUMO CUT” is the sample following the addition of SUMO protease. “RALF8” is from the purified protein. The final yield of purified RALF8 was usually 3.5 mg/L minimal medium.
