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miR-495 regulated cartilaginous degeneration-related molecules, including MMP-1, MMP-13, COL II and COL X. Chondrocytes and HC cells were treated with miR-495-mimic or miR-495-inhibitor for 24 h. Concentrations of secreted MMP-1 (A), MMP-13 (B), COL II (C) and COL X (D) in the media of cells were measured by ELISA assay. Data represent the mean ± SD of three independent experiments. ***, P < 0.001; **, P < 0.01; *, P < 0.05 (one-way ANOVA with Tukey’s post hoc test).
