Figure 5.

AKT1 overexpression affected miR-495-mimic-induced cell apoptosis and growth inhibition. A. SW1353 cells were treated with miR-495-mimic at indicated time points. Indicated protein level was analyzed by western blot assay. B. SW1353 cells transfected with AKT1 were treated with miR-495-mimic. Indicated protein level was analyzed by western blot assay. C. The indicated cells with or without AKT1 transfection were treated with miR-495-mimic for 24 h. The protein level of cleaved caspase-3 was measured by western blot assay. D. The indicated cells with or without AKT1 transfection were treated with miR-495-mimic for 72 h. Cell proliferation measured with MTT kit. Data represent the mean ± SD of three independent experiments. **, P < 0.01 (one-way ANOVA with Tukey’s post hoc test). E. The indicated cells with or without AKT1 transfection were treated with miR-495-mimic for 24 h. Percentage of wound closure measured on the basis of scratch wound assay. The wound healing assay was expressed as relative wound width. The results were expressed as the means ± SD of three independent experiments. **, P < 0.01; *, P < 0.05 (Two-way ANOVA with Tukey’s post hoc test).