Figure 3.

TP73-AS1 epigenetically inhibits p21 through binding with EZH2. A. The subcellular location of TP73-AS1 in the nuclear and the cytoplasm fraction. B. The level of CDK inhibitors (CKIs), including p15, p16, p21, p27, p57. C. Western blot revealed the p21 protein in the TP73-AS1 silencing transfection. D. RNA-binding protein immunoprecipitation (RIP) assay showed the binding of TP73-AS1 with EZH2. E. Chromatin immunoprecipitation (ChIP) revealed the direct bound of EZH2 with p21 promoter region. F. Kaplan-Meier survival curve analysis based on the TCGA database suggested the prognosis of EOC patients with higher or lower group. **< 0.01.