Figure 1.

HBSP pretreatment alleviates cardiomyocytes H/R injury. A. Cells were treated with different concentration of HBSP and cell viability was detected using CCK-8 kit. B. Cells were cultured with HBSP and the supernatant was collected to detect LDH activity. C. The relative expression of LC3B performed by Western blot. D. Cells were pretreated with HBSP, and then infected with GFP-LC3. Representative photos were shown in the upward side and cells with GFP-LC3 puncta were qualified in the downward. E. Representative images and quantitative of apoptosis was performed by TUNEL assay. F. Western blot analysis of cleaved caspase 3 after treated with HBSP. Data represent the mean ± SD. *P < 0.05, **P < 0.01 vs. Control group. #P < 0.05, ##P < 0.01 vs. H/R group.