Figure 2.

HBSP affects cell autophagy and apoptosis by regulating miR-21. A. Relative expression of miR-21 in H9C2 cell was detected by qRT-PCR. B. Cells were transfected with miR-NC or miR-21 mimic and the expression of miR-21 was detected by qRT-PCR. C. CCK-8 was performed when cells transfected with miR-NC or miR-21 mimic. D. HBSP pretreatment and miR-21 mimic transfection inhibits fluorescence puncta accumulations of GFP-LC3. E, F. Representative Western blot and quantitative evaluation of LC3B and cleaved caspase 3. G. Representative images and quantitative of induction of apoptosis performed by flow cytometry analysis after HBSP pretreatment and miR-21 mimic transfection. Data represent the mean ± SD. *P < 0.05, **P < 0.01 vs. Control or H/R (miR-NC). #P < 0.05 vs. H/R+HBSP (miR-NC).