Figure 4.

Knockdown of Atg12 enhances the inhibition autophagy and apoptosis effect of HBSP. A. The transfection efficiency of Atg12 was determined by qRT-PCR. B. Cells were transfected with si-NC or si-Atg12 to detect the mRNA level of Atg12. C. HBSP pretreatment enhances the effect of knockout Atg12 on the expression of autophagy proteins Atg12, Atg5-Atg12, LC3B and P62. D. Cell apoptosis was assessed by flow cytometry assay and the experiment was repeated three times for the calculation of apoptosis rate. E. Cleaved caspase 3 was detected using Western blot. Data represent the mean ± SD. *P < 0.05, **P < 0.01 vs. Control or H/R (si-NC). #P < 0.05 vs. si-NC or H/R+HBSP (si-NC).