Figure 3.
HOXD10 was a direct target of miR-501. (A) Binding site of miR-501 and the 3’-UTR of HOXD10. (B) Luciferase activity in cells transfected with the miR-501 inhibitor or NC-miR and wt-HOXD10 or mt-HOXD10. (C) HOXD10 expression in cells transfected with miR-501 inhibitor or NC-miR was analyzed by Western blot analysis. (***P<0.001, ns: not significant) miR-501: microRNA-501; HOXD10: homeobox D10; UTR: untranslated region; wt: wild-type; mt: mutant; NC-miR: negative control miRNA.