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. 2019 Apr 15;11(4):2042–2055.

Figure 3.

Figure 3

GA induces autophagy in LPS-induced RAW264.7 cells. A-D. The levels of LC3-II/I, Beclin-1, and SQSTM1/P62 were detected by Western blotting in RAW264.7 cells, and the bar graphs showed the quantitative analysis results of the corresponding protein in each group, respectively. E. The representative images of the fluorescence of LC3 in RAW264.7 cells (magnification ×63). F. The remarkable images of autophagosomes ultrastructure by TEM in RAW264.7 cells (magnification ×1500, ×6000, respectively). G. Quantitative analysis of autophagosomes numbers in each group, and autophagosomes were indicated shown by the red arrows. The experiments were performed four independent times (n=4) and bars represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 in comparison to control group. # P < 0.05, ## P < 0.01, ### P < 0.001 in comparison to LPS group. &&& P < 0.001 versus the GA + LPS group.