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. 2019 Apr 15;11(4):2603–2615.

Figure 4.

Figure 4

Smad4 and JAK1 knockdown inhibits osteogenic differentiation of hADSCs. A. Smad4 and JAK1 mRNA expression levels examined via qRT-PCR at different time points during osteogenic differentiation of hADSCs; B-D. Smad4 and JAK1 mRNA and protein expression level examined via qRT-PCR and Western blot following siRNAs transfection in hADSCs; E-O. Osteogenic marker protein expression examined via Western blot at day 14 after Smad4 and JAK1 knockdown; P. ALP staining and Alizarin Red staining at day 14 showed inhibited ALP activity and calcification following Smad4 and JAK1 knockdown when compared with siNC group (×10). Q. hADSCs proliferation was determined by direct cell counting after the treatment of Smad4 and JAK1 knockdown at different time points. *P < 0.05 compared with non-targeting siRNA (si-NC) group.