Figure 2.

AB4 induces SMMC7721 cell apoptosis. A. Representative images of DAPI, Annexin V-FITC and PI fluorescence staining. Scale bar = 10 μm. B. Flow cytometry analysis of apoptosis after treatment with 80 μM AB4 or vehicle control. C. SMMC7721 cells were treated with 20, 40 and 80 μM AB4 for 24 h. Western blot analysis was performed to evaluate the expression of apoptosis-related proteins. D. Grayscale analysis of Bcl-2, Bax, Cleaved Caspase-3 and Cleaved PARP after indicated treatment. *P < 0.05, **P < 0.01 versus vehicle control group.