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AB4 inhibits the PI3K/Akt/mTOR pathway signaling. A. SMMC7721 cells were treated with 20, 40 and 80 μM AB4 for 24 h. Phosphorylation of Akt and mTOR was determined by Western blot analysis. B. Grayscale analysis of Akt and mTOR phosphorylation after AB4 treatment. *P < 0.05, **P < 0.01 versus vehicle control group.
