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. 2019 Apr 15;11(4):2219–2231.

Figure 2.

Figure 2

Effects of miR-147 on expression of TLR-7 by suppression or gain of miR-147 function in Rat macrophagic cells. (A) Results of RT-qPCR for expression of miR-147. (B) RT-qPCR results for expression of TLR-7 mRNA and (C) Results are immunoblotting studies for protein expression of TLR-7 after miR-147 mimics or inhibitor was transfected into rat macrophagic cells for 48 h. (D) Immunoblotting results for TLR-7 protein expression after transfection with miR-147 mimics into rat macorphagic cells for 48 h. (E) Results of Immunoblotting studies for protein expression of TLR-7 and (F) Results of RT-qPCR for expression of mRNA expression of IFN-β and TNF-α in rat macrophagic cells and results of ELISA for protein expression of TNF-α in cell supernatant. (G) The cell supernatant incubated with with miR-147 mimics and inhibitors for 24 h and stimulation of R-848 (R-848, TLR-7 ligand) for next 24 h. GAPDH i.e glyceraldehyde-3-phosphate dehydrogenase was selected as loading standard control in RT-qPCR. *, P<0.05 compared to NC; #, P<0.05 compared to control (Mann-Whitney U-test).