Figure 5.

Expression of miR-147 and TLR-7 in PS induced arthritis rats (PIA) with or without receiving treatment of methotrexate (MT). (A) Score for arthritis and (B) Perimeter of rat foot-pad (mm) in PIA rats with or without treatment of MT. (C) Results of RT-qPCR for expression of TLR-7 mRNA and miR-147 in rat spleens rats of control group, saline-treated PS and MT treated PIA rats group on day 20 after PS injection. GAPDH i.e. glyceraldehyde-3-phosphate dehydrogenase was used as loading control standard *, P<0.05 compared to control, #, P<0.05 compared to PS + MT treated rats, **, P<0.01 and ***, P<0.001 compared to control group rats.