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Transduction of human glioma cell U251 with Ad-shSKP2 (shSKP2 group) and Ad-shNC (shNC group). A. At 24 hours after transduction, the efficiency was examined by counting GFP-positive cell under microscope. B. At 48 hours after transduction, the down-regulation of SKP2 in shSKP2 group was examined using Western blotting assays. Shown is the representative set of data from 3 independent experiments.
