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. 2019 Apr 15;11(4):2496–2506.

Figure 2.

Figure 2

Vitamin D reduces inflammation in the intervertebral disc. (A) Results of protein expression of IL-1β and TNF-α in four groups was determined by Western blotting. β-Actin was used as an internal control. (B) Results of mRNA expression of IL-1β, IL-6 and TNF-α in four groups were determined by real time PCR. In addition, The protein expression of IL-1β (C) and TNF-α (D) was measured by Elisa. (“*” means there is a statistical difference with the control group and “#” means there is a statistical difference with the vehicle group. N number is all 3. IL-1β: vehicle-control. P = 0.0003; low dose-vehicle, P = 0.05; high dose-vehicle, P = 0.00156. TNF-α: vehicle-control. P = 0.00285; low dose-vehicle, P = 0.01023; high dose-vehicle, P = 0.00055).