Figure 2.

Bacterial RNase III cleaves long dsRNA at preferred sites. (A) Schematic representation of the in vitro cleavage experiment. (B) dsRNA of FF-luc sequence was cleaved by either Aquifex aeolicus or Escherichia coli RNase III. The cleavage products were separated on 20% polyacrylamide non-denaturing (native) gels and detected by staining. Synthetic single-stranded RNAs with certain length were used as markers. After next-generation sequencing, reads of cleavage products were mapped back to (C) FF-luc or (D) MBP, respectively. Coverage plots are presented.