Figure 10.
Tubercidin causes an mRNA export impairment even when SGs cannot assemble due to a GSK inhibitor. U2OS cells treated with a GSK inhibitor for 3 h before dox induction for 6 h with or without tubercidin addition. The export of the E6 mRNA, detected by RNA FISH (black), is impaired in tubercidin cells even when SGs (magenta) do not form due to GSK. SGs are labeled with antibodies to G3BP1. Bar = 10 μm.