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. 2019 Mar 27;47(9):4638–4651. doi: 10.1093/nar/gkz187

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Expression of cat reporter gene fused to different DNA fragments derived from the nusA-infB operon. (A) Representation of the cat transcriptional fusion constructs used in this study. The DNA fragments schematically indicated in the figure, each containing chromosomal regions corresponding to the different promoters, were cloned upstream of promoter-less cat gene in pKK232–8; (B) Levels of cat mRNA expressed at 37°C in cells harbouring plasmids carrying the transcriptional fusions shown in panel (A) and containing the indicated promoters; (C) Relative increase of the steady-state levels of cat mRNA present in cells carrying the constructs containing all three promoters (P-1, P0 and P2) (•) or only P-1 (□), P0 (△) and P2 (▴) at the times after cold shock reported in the abscissa. The RNA level at time zero is taken as = 1.