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. 2019 Mar 27;47(9):4694–4706. doi: 10.1093/nar/gkz186

Figure 1.

Figure 1.

Sgs1 binds to RPA-coated ssDNA. (A) ATP hydrolysis assays with 0, 0.25, 0.8, 2.4 μM RPA with unlabeled Sgs1. The data points represent the mean and standard deviation of three independent experiments. (B) Schematic of ssDNA curtain assay used to measure the binding and translocation activity of GFP–Sgs1 on ssDNA-RPA molecules. (C) Widefield images showing a ssDNA bound by RPA–mCherry (magenta) and GFP–Sgs1 (green). (D) Binding distribution of GFP–Sgs1 on ssDNA bound by RPA–mCherry molecules, error bars were generated by bootstrapping the data using a custom python script (N = 340).