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. 2019 Feb 14;47(9):4751–4764. doi: 10.1093/nar/gkz100

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — The N-terminal extension is crucial for DXO1 function in chloroplast-related processes. (A) Two-week-old dxo1-2 transgenic plants. (B) RT-qPCR of selected mRNAs in dxo1-2 and dxo1-2 expressing DXO1 variants. Gene identifiers are indicated below the graph. Results are the mean of three independent biological replicates with error bars representing standard deviation; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 (t-test). (C) Small RNA detection by northern blotting; siRNA accumulation from sRNA-seq (log₂FC) is indicated; 5S rRNA, loading control. (D) Activity toward NAD⁺-capped RNA substrate in the presence of PAP. Reaction products after 30 min incubation were resolved by 8% urea-PAGE. See Supplementary Figure S6 for additional information.