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. 2019 Mar 6;47(9):4569–4585. doi: 10.1093/nar/gkz145

Figure 3.

Figure 3.

UNG expression in CIT-stimulated and unstimulated CH12F3 cells. (A) Cell growth (upper panel) and UDG activity (lower panel) in untreated and stimulated (+CIT) CH12F3 cells (WT). 200 000 cells/ml were seeded and samples were harvested for cell counting and UDG activity assays at several time points (0–96 h). UDG activity values represent the mean of five replicates at each time point. Standard errors are indicated on the curves. (B) Detection and relative quantitation of AID and UNG in untreated and stimulated (+CIT) CH12F3 cells cultures. Cells were seeded and stimulated as in A. At each time point AID (upper panel) and total UNG (lower panel) were quantified by targeted mass spectrometry. The data represent the mean of two biological replicates. All data are normalized to the protein level at the time of seeding (0 h). (C) Ung1 and Ung2 gene expression measured by RT-qPCR. The curves represent fold changes in gene expression in stimulated (+CIT) versus untreated cell cultures (+CIT/untreated). Values were calculated by the ΔCt method using untreated cells at each time point as reference. Each value represents the mean of two technical replicates. Aicda and Gapdh were included as positive and negative control genes, respectively.